Spain dominated the game but the Russians were able to hold on till Extra-time and then penalties which they went on to win.
Koke missed a spot kick as did Iago Aspas to give Russia the win and now the hosts are to face Croatia in the quarter-finals for the first time in their history.
He said: “It’s a reality that this is my last game with the national team, on a wonderful stage,” Iniesta told reporters after the defeat.
“A marvellous spell is over. Sometimes the end is not as you dreamed it.
“The national team will continue to be successful because of the level of players we have and that is what they should try.”
“It would be an advantage to say that Lopetegui’s thing has to do with the result, in the end those of us who have the ball are the players,” he continued.
“The main culprits in football are the players that are the ones on the field.”
Iniesta played a total of 131 times for Spain, scoring on 13 occasions in national team colours including the goal that won them the World Cup in 2010.
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